Two types of resequencing, four kinds of input data :

    ►Exon-centered resequencing

• Track1: cDNA sequence/ID only ( +  UCSC goldenpath chromosome)

                 If you have only mRNA IDs (Genbank accession or gi numbers) or mRNA sequences, please select this one!

                Attention: Human genome sequence only!

• Track2: user's own gene annotation generated by DNannotator + user's own gDNA

                 If you have a feature table for exon annotation generated by DNannotator, please select this one!

• Track3: annotated sequence data from UCSC

                 If you have the exon and flanking sequences downloaded from UCSC , please select this one!

Track 3 is preferred if the gene is annotated on UCSC map, as the promoter region sequence can be extracted there too.  Otherwise, user may need to manually define & extract promoter region sequence.

    ►Resequencing across the genomic regions

• Track4: user's own continuous gDNA

                 If you need to design assays to screen continuous gDNA sequence, regardless of exon/intron structure, please select this one!