1. * List of dbSNP rs IDs  (must be prefixed with "rs", as rs125, one ID per line) or SNPs with flanking sequence in specified format :  (Temporarily, if rs IDs are used as input, only 20 or less SNPs could be processed each time due to BLAT restriction at Genome Browser. No such restriction apply for the input of formatted sequences longer than the maximum PCR product size. SNPSequer for SNP sequence preparation is under renovation to remove this restriction. )

or upload data file

 Input data type: rs list snp list (Note: data can not be processed without correct data type identification. "SNP sequences data" is preferred for efficient data processing.)

 Additional flanking sequences can be extracted by using FastMode MediumMode SlowMode , if the SNP flanking sequence was too short in the supplied data.

   (MediumMode or SlowMode is suggested for SNPs that failed to work with FastMode.  Refer to SNP mapping function for the meaning of different types of modes.)

2. Output filename label: (Please use one word comprised of only alphabets)

3. * Restriction Enzyme (RE) list:

    REBASE enzymes
    Inexpensive enzymes (<$0.13/unit)
    Efficient/reliable enzymes (used in publications)
    User's own restriction enzyme list (format identical to the above restriction enzyme list):
    

4. * For mismatched PCR-RFLPs, a base perfect match is required at the 3' end of the primer.

5. Adjustable parameters for Primer 3:    (Primer3's internal parameters use the default value)

   1）For natural PCR-RFLPs:

• PCR product size range (amplicon size will be tried for 301-500 first, then 501-700, 701-1000... until a suitable one is found)
• Primer size: optimal:    min:   max: bp
• Primer Tm: optimal:    min:   max: bp
• Primer GC%: optimal:   min:   max: bp
• Maximum primer Tm difference:
• Max Self Complementarity:
• Max 3' Self Complementarity:
• Maximum number of polyX in primer: (for example, polyT, polyC)
• Candidate primer pairs for each SNP: 1 2 3 4 5

  2）For mismatch PCR-RFLPs:

• PCR product size range   (the same format as above)
• Primer size: optimal:    min:   max: bp
• Primer Tm: optimal:    min:   max: bp
• Primer GC%: optimal:   min:   max: bp
• Maximum primer Tm difference:
• Max Self Complementarity:
• Max 3' Self Complementarity:
• Maximum number of polyX in primer: (for example, polyT, polyC)
• Candidate primer pairs for each SNP: 1 2 3 4 5

6. Choice of outputs:

• SNPs, amplicons, fragments and primers data in tabular text
• Detailed data file
• Primer3 raw data

7. Email address to which the results will be mailed to:

Email address * 

8. Choice of gzip compression for output data: yes, zip it no, original data please

9. Choice of email: Emails with result data attached Web Display Email without attachment. Only notice of URLs to access results

Email attachment size limit: 1 MB 2 MB 4 MB 6 MB

Check this box , if your input data file is from a Mac machine,