Single sequence BLAST against single sequence
1. Please give the query sequence data file (one sequence in FASTA format):
2. Please give the subject sequence data file (one sequence in FASTA format):
Additional parameters to set:
Program blastn: NA vs NA blastp: Prot vs Prot blastx: NA vs Prot. tblastx: Prot vs translated NA db tblastn: NA vs translated NA db
Filter query sequence Use filter No filter
E value (the lower the value, the more stringent the cutoff. NCBI default 10. If analyzing short fragments like oligo, use 10 or higher.)
Gap extension penalty (BLAST default is 2)
Output name you prefer (The file name should not contain white space. It should be only composed of numbers, letters a to Z, "-" and "_" .)
First filter
@ Minimum size of match for valid hits bp (If your SNP is with shorter flanking sequences, please decrease this number accordingly) @ Minimum percentage of identities for valid hits 20% 30% 40% 50% 60% 70% 80% 90% 95% 96% 97% 98% 99% 100%
@ Minimum size of match for valid hits bp (If your SNP is with shorter flanking sequences, please decrease this number accordingly)
@ Minimum percentage of identities for valid hits 20% 30% 40% 50% 60% 70% 80% 90% 95% 96% 97% 98% 99% 100%
3. Output Choices:
4. The BLAST results in one file will be delivered to:
Email address
Your email box accepts message size limit of 1 MB 2 MB 4 MB 6 MB per message
choice of output in gzip compressed format: yes, zip it no, original data please
If your input data file is from a Mac machine, please be sure to check this box
