Batch sequences BLAST against NCBI database
1. Please copy & paste in the query sequence data (batch sequences in FASTA format):
or upload file
2. Search against NCBI database nr htgs est est_human est_mouse month gss
Additional parameters to set:
Program blastn: NA vs NA blastp: Prot vs Prot blastx: NA vs Prot. tblastx: Prot vs translated NA db tblastn: NA vs translated NA db
Filter query sequence Use filter No filter
E value (the lower the value, the more stringent the cutoff. NCBI default 10. If analyzing short fragments like oligos, use 10 or higher.)
Gap extension penalty (BLAST default is 2)
Output name you prefer (The file name should not contain white space. It should be only composed of numbers, letters a to Z, "-" and "_" .)
First filter
@ Minimum size of match for valid hits bp (If your SNP is with shorter flanking sequences, please decrease this number accordingly) @ Minimum percentage of identities for valid hits 20% 30% 40% 50% 60% 70% 80% 90% 95% 96% 97% 98% 99% 100%
@ Minimum size of match for valid hits bp (If your SNP is with shorter flanking sequences, please decrease this number accordingly)
@ Minimum percentage of identities for valid hits 20% 30% 40% 50% 60% 70% 80% 90% 95% 96% 97% 98% 99% 100%
3. Output Choices:
4. The BLAST results in one file will be delivered to:
Email address
Your email box accepts message size limit of 1 MB 2 MB 4 MB 6 MB per message
choice of output in gzip compressed format: yes, zip it no, original data please
If your input data file is from a Mac machine, please be sure to check this box
