ePCR-based STSs annotation
NOTE: revised ePCR, primarily designed by Gregory Schuler, is used.
* entries are required
1.* Please give the STS sequence data file (may contain all the STSs in specified format ):
or use search for all STSs in the database human UniSTS Genethon marker
2. Please give the gDNA sequence FASTA data file.
*
3. *Please give the description of the un-annotated target genomic DNA sequence, or Genbank data file or Genbank header file (.gbheader produced by previous DNannotator run).
You may check this box if this is your first time to run annotation for this sequence on DNannotator. Then, please fill in the following information but no Genbank file will be needed. A Genbank header file will be created for you based on data from FASTA sequence and the information you provided here.
Sequence description: Chromosome: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 X Y
Un-annotated Genbank file (should be the one generated by DNannotator in previous analysis):
4. STS PCR product size difference range
5. Choice of outputs:
Annotation Genbank format data or Genbank format features
viewable in Vector NTI or Artemis (standard Genbank format)
Feature table ready for database in tab-delimited format
Duplicated features
ePCR original output
Original full-length Genbank format data (with sequence body)
Original gbheader
6. Please specify the email address where the final annotated sequence can be delivered:
Email address *
choice of output in gzip compressed format: yes, zip it no, original data please
Your email box accepts message size limit of 1 MB 2 MB 4 MB 6 MB per message
If your input data file is from a Mac machine, please be sure to check this box